核酸酶 S7
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产品编号:B004797 品牌 : Diamond CAS号: |
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名称,核酸酶 S7,
英文名称;Nuclease, Micrococcal,
级别;Activity ≥6,000 U/mg protein
储存;冷冻(-20℃)
相关类别;生化试剂
概述;Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
技术规格
Source | Staphylococcus aureus (Strain ATCC #27735) |
Description | Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder. |
Storage | Store at 2-8°C. |
Activity | ≥6,000 units per mg protein |
Unit Definitions | One Unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate. |
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