Anti-β-actin Mouse Monoclonal Antibody 抗β-actin单克隆抗体

产品属性

β-Actinis one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actinare useful as loading controls for Western Blotting. However it should be noted that levels of β-Actinmay not be stable in certain cells. For example, expression of β-Actinin adipose tissue is very low and therefore β-Actinshould not be used as loading control for these tissues.

Product Name:β-Actinmouse monoclonal antibody

Clone Number: 6B8

Isotype: IgG1

Immunogen: Full-length β-Actin protein of human.

Tested applications: WB   IHC   IF

Recommended dilutions: WB: 1:5000-2000  IHC: 1:200-100  IF: 1:200-100

Optimal dilutions/concentrations should be determined by the end user.

Molecular Weight: 43KD

Form:Liquid

Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol

Storage: β-Actin antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Reactivity:Human, Monkey, Mouse, Rat, hamster, Dog, Spodoptera frugiperda, chicken, Zebrafish

Western blot analysis of Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with β-Actin mouse mAb(6B8) diluted at 1:5000.

Immunohistochemistry Staining of Human ovary tissue with β-Actin mouse mAb(6B8) diluted at 1:200.